Universidad de Cordoba 1 article published in JoVE Biology Isolation and Characterization of Cyanobacterial Extracellular Vesicles Steven J. Biller*1, María del Carmen Muñoz-Marín*2, Steeve Lima3,4,5, Jorge Matinha-Cardoso3,4, Paula Tamagnini3,4,6, Paulo Oliveira3,4,6 1Department of Biological Sciences, Wellesley College, 2Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario, Universidad de Córdoba, 3i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 5MCbiology Doctoral Program, ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 6Departamento de Biologia, Faculdade de Ciências, Universidade do Porto The present protocol providesdetailed descriptions for isolation, concentration,and characterization of extracellular vesicles from cyanobacterial cultures. Approaches for purifying vesicles from cultures at different scales, trade-offs among methodologies, and considerations for working with field samples are also discussed.
