Universite; Paris Sud View Institution's Website 4 articles published in JoVE Immunology and Infection Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells Yasmine Smail1,2,3,4, Chunhui Sun1,2,3,4, Laurent Combettes5, Guy Tran Van Nhieu1,2,3,4 1Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB), Collège de France, 2Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, 3Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, 4MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, 5Inserm, UMRS1174, Université Paris Sud Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized. Bioengineering Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis Yanxia Hou1, Maria Genua1, Laurie-Amandine Garçon1, Arnaud Buhot1, Roberto Calemczuk1, David Bonnaffé2, Hugues Lortat-Jacob3, Thierry Livache1 1Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble, 2Institut de Chimie Moléculaire et des Matériaux d'Orsay, Université Paris-Sud, 3Institut de Biologie Structurale A novel approach is described for construction of electronic tongue (eT), which greatly simplifies the design and production of sensing materials, and allows the eT to generate continuous evolution profiles and landscapes for samples in liquid. The obtained eT is efficient for common protein analysis such as discrimination. Biology Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS) Catherine Marquer1, Sandrine Lévêque-Fort2,3, Marie-Claude Potier1 1Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, 2Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, 3Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition. Neuroscience Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals Romain Chery1, Barbara L'Heureux1, Mounir Bendahmane1, Rémi Renaud1, Claire Martin1, Frédéric Pain1, Hirac Gurden1 1Laboratoire d’Imagerie et de Modélisation en Neurobiologie et Cancérologie, UMR8165 Université Paris Sud 11, Paris Diderot 7 – CNRS This article presents the protocols of intrinsic optical signals and flavoproteins autofluorescence signals imaging to map odor-evoked activities at the surface of the olfactory bulb in mice.