Centre Hospitalier Lyon-Sud 2 articles published in JoVE Cancer Research Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up Thomas Boyer1,2, Fanny Gonzales2,3, Adriana Plesa4, Pauline Peyrouze2, Adeline Barthelemy2, Soizic Guihard2, Bruno Quesnel2,5, Christophe Roumier1,2, Claude Preudhomme1,2, Meyling Cheok2 1Laboratoire d’hématologie, CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille We outline a protocol to detect simultaneous expression of leukemia stem cell markers on primary acute myeloid leukemia cells by flow cytometry. We show how to quantify three progenitor populations and a putative LSC population with increasing degree of maturation. We confirmed the presence of these populations in corresponding patient-derived-xenografts. Cancer Research Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay Jean-Baptiste Guy*1,2, Sophie Espenel*1,2, Alexis Vallard1,2, Priscillia Battiston-Montagne1, Anne-Sophie Wozny1,3, Dominique Ardail1,3, Gersende Alphonse1,3, Chloé Rancoule1,2, Claire Rodriguez-Lafrasse1,3, Nicolas Magne1,2 1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud This study reports two different methods for the analysis of cell invasion and migration: the Boyden chamber assay and the in vitro video microscope-based wound-healing assay. The protocols for these two experiments are described, and their benefits and disadvantages are compared.