Universite Lyon 2 articles published in JoVE Neuroscience Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons Arnaud Jacquier1,2, Valérie Risson1, Laurent Schaeffer1,3 1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217, Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire, Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection. Neuroscience Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo Céline Delloye-Bourgeois*1, Arnaud Jacquier*1, Julien Falk1, Valérie Castellani1 1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.
