Universite Aix-Marseille 6 articles published in JoVE Medicine Induction and Phenotyping of Acute Right Heart Failure in a Large Animal Model of Chronic Thromboembolic Pulmonary Hypertension David Boulate1, Myriam Amsallem1,2, Jean-Baptiste Menager1, Simon Dang Van1, Peter Dorfmuller3, Andrew Connolly4, Alban Todesco5, Benoit Decante1, Elie Fadel1, Francois Haddad2, Olaf Mercier1 1Research and Innovation Unit, RHU BioArt Lung 2020, Marie Lannelongue Hospital, 2Phenotypic and Biomarker Core Laboratory, Cardiovascular Institute, Stanford University, 3Department of pathology, Marie Lannelongue Hospital, 4Department of pathology, UCSF School of Medicine, 5Department of thoracic surgery, Hopital Nord, APHM, Aix-Marseille University We present a protocol to induce and phenotype an acute right heart failure in a large animal model with chronic pulmonary hypertension. This model can be used to test therapeutic interventions, to develop right heart metrics or to improve the understanding of acute right heart failure pathophysiology. Behavior Highlighting and Reducing the Impact of Negative Aging Stereotypes During Older Adults' Cognitive Testing Marie Mazerolle1, Isabelle Régner2, François Rigalleau3, Pascal Huguet4 1Department and Laboratory of Psychology, MSHE, Université Bourgogne Franche-Comté, 2Aix-Marseille University, CNRS, LPC, 3Department of Psychology, University of Poitiers, CNRS, 4University of Clermont Auvergne, CNRS, LAPSCO Here, we present a protocol designed to show how negative aging stereotypes can impair memory performance of older adults during cognitive testing and how to reduce this deleterious effect. This method can help older people to perform at an optimal level during testing in both lab studies and clinical settings. Engineering Preparing a Celadonite Electron Source and Estimating Its Brightness Evelyne Salançon1, Alain Degiovanni1, Laurent Lapena1, Roger Morin1 1CINaM-UMR7325, Aix-Marseille University, CNRS The article presents a protocol to prepare a celadonite source and estimate its brightness for use in a long-range imaging low-energy electron point-source projection microscope. Developmental Biology Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues Imen Jebeniani1, Julia Leschik2, Michel Puceat1 1INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, 2Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos. Immunology and Infection Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics Sreekanth Dasari1, Pascal Weber1, Camélia Makhloufi1, Erica Lopez2, Claire-Lise Forestier1 1INSERM 1095, URMITE, University of Aix-Marseille, 2U.F.R de Médecine, University of Aix-Marseille This article reports on a detailed method for the dynamic measurement and quantification of blood flow velocity within individual blood vessels of the mouse liver vasculature using intravital microscopy imaging in combination with a specific methodology for image acquisition and analysis. Biology Cell Labeling and Injection in Developing Embryonic Mouse Hearts Emilye Hiriart*1, Patrick van Vliet*2, Ralf J. Dirschinger2, Sylvia M. Evans2, Michel Puceat1 1INSERM UMR-910, Aix-Marseille University, 2Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.
