Universite de Bourgogne 5 articles published in JoVE Behavior Highlighting and Reducing the Impact of Negative Aging Stereotypes During Older Adults' Cognitive Testing Marie Mazerolle1, Isabelle Régner2, François Rigalleau3, Pascal Huguet4 1Department and Laboratory of Psychology, MSHE, Université Bourgogne Franche-Comté, 2Aix-Marseille University, CNRS, LPC, 3Department of Psychology, University of Poitiers, CNRS, 4University of Clermont Auvergne, CNRS, LAPSCO Here, we present a protocol designed to show how negative aging stereotypes can impair memory performance of older adults during cognitive testing and how to reduce this deleterious effect. This method can help older people to perform at an optimal level during testing in both lab studies and clinical settings. Neuroscience Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor David Jarriault1, Xavier Grosmaitre1 1UMR Centre des Sciences du Goŭt et de l'Alimentation, CNRS, INRA, Université de Bourgogne Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors. Neuroscience Testing Drosophila Olfaction with a Y-maze Assay Mégane M. Simonnet1,2,3, Martine Berthelot-Grosjean1,2,3, Yael Grosjean1,2,3 1Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, 2Centre des Sciences du Goût et de l'Alimentation, UMR-1324 INRA, 3Centre des Sciences du Goût et de l'Alimentation, Université de Bourgogne We present a choice test to reveal the influence of odorants on Drosophila behavior using a Y-maze assay. Engineering Evaluating Plasmonic Transport in Current-carrying Silver Nanowires Mingxia Song1, Arnaud Stolz1, Douguo Zhang2, Juan Arocas1, Laurent Markey1, Gérard Colas des Francs1, Erik Dujardin3, Alexandre Bouhelier1 1Laboratoire Interdisciplinaire Carnot de Bourgogne CNRS-UMR 6303, Université de Bourgogne, 2Department of Optics and Optical Engineering, University of Science and Technology of China, 3CEMES, CNRS-UPR 8011 Silver nanowires can simultaneously transport electrons and optical information in the form of surface plasmons. A procedure is described here to realize such a shared circuitry and the limitations at propagating both information carriers are evaluated. Neuroscience Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing Reema P. Vazirani1, Xavier Fioramonti2, Vanessa H. Routh1 1Department of Pharmacology and Physiology, Rutgers New Jersey Medical School, 2Center for Taste and Feeding Behavior, Universite de Bourgogne The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.
