INSERM 6 articles published in JoVE Cancer Research In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research Jennifer C. Wischhusen1, Katheryne E. Wilson2 1Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Centre Léon Bérard, 2Department of Radiology/Molecular Imaging Program at Stanford, School of Medicine, Stanford University The in vivo immunofluorescence localization (IVIL) method can be used to examine in vivo biodistribution of antibodies and antibody conjugates for oncological purposes in living organisms using a combination of in vivo tumor targeting and ex vivo immunostaining methods. Neuroscience Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices Fanny Etienne1,2,3, Vincenzo Mastrolia1,2,3, Luc Maroteaux1,2,3, Jean-Antoine Girault1,2,3, Nicolas Gervasi*1,2,3, Anne Roumier*1,2,3 1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice. Cancer Research A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model Alexia Mopin1, Virginie Driss1, Carine Brinster1,2 1Institut pour la Recherche sur le Cancer de Lille (IRCL), INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining. Medicine Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease Ophélie Vacca1,2,3, Brahim El Mathari1,2,3, Marie Darche1,2,3, José-Alain Sahel1,2,3, Alvaro Rendon1,2,3, Deniz Dalkara1,2,3 1Department of Therapeutics, Institut de la Vision, Sorbonne Universtés, UPMC Univ Paris 06, UMR_S 968, 2INSERM, U968, 3CNRS, UMR_7210 To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability. Immunology and Infection Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells Célia Parinot1,2,3, Quentin Rieu1,2,3, Jonathan Chatagnon1,2,3, Silvia C. Finnemann4, Emeline F. Nandrot1,2,3 1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968, Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation, Fordham University This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C. Bioengineering Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo Nihal E. Vrana1, Agnes Dupret-Bories1,2, Christophe Chaubaroux1, Elisabeth Rieger1,2, Christian Debry1,2, Dominique Vautier1,3, Marie-Helene Metz-Boutigue1,3, Philippe Lavalle1,3 1Biomatériaux et Bioingénieriee, INSERM, 2Service Oto-Rhino-Laryngologie, Hôpitaux Universitaires de Strasbourg, 3Faculté de Chirurgie Dentaire, Université de Strasbourg In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.
