CNRS UMR 7225 1 article published in JoVE Neuroscience Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo Tristan Altwegg-Boussac1,2,3,4, Séverine Mahon1,2,3,4, Mario Chavez1,2,3,4, Stéphane Charpier1,2,3,4, Adrien E. Schramm1,2,3,4 1Inserm U1127, 2CNRS UMR 7225, 3UPMC Univ Paris 06, UMR S 1127, Sorbonne Universités, 4Institut du Cerveau et de la Moelle épinière (ICM) This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.
