INRA 2 articles published in JoVE Biology Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli Qiaojing Li1, Charles-Adrien Richard1, Mohammed Moudjou1, Jasmina Vidic1 1Virologie et Immunologie Moléculaires, INRA A two-step chromatographic method is described for the purification of recombinant Shadoo protein expressed as inclusion bodies in Escherichia coli, as well as a protocol to fibrillate purified Shadoo into amyloid structures. Biology Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland Edith Honvo-Houéto1, Sandrine Truchet1 1Jouy-en-Josas Centre, INRA The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.
