Centre Leon Berard 3 articles published in JoVE Biology A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts Kawtar Arizkane1,2,3, Kevin Geistlich1,2,3,4, Laurine Moindrot1,2,3, Emma Risson1,2,3, Sandrine Jeanpierre1,2,3,4, Léa Barral1,2,3, Anaëlle Bobard1,2,3, Giulio Menegazzi5, Thibault Voeltzel1,2,3, Véronique Maguer-Satta1,2,3, Sylvain Lefort1,2,3 1Centre de Recherche en Cancérologie de Lyon, 2Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Centre Léon Bérard, 5Department of Experimental and Clinical Biomedical Sciences Mario Serio, Università degli Studi di Firenze Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events. Cancer Research Evaluation of the Feasibility, Safety, and Accuracy of an Intraoperative High-intensity Focused Ultrasound Device for Treating Liver Metastases Aurélien Dupre1,2, David Melodelima2, David Perol3, Yao Chen1, Jérémy Vincenot2, Jean-Yves Chapelon2, Michel Rivoire1,2 1Department of Surgical Oncology, Centre Léon Bérard, 2Applications des ultrasons à la thérapie (LabTAU), Institut national de la santé et de la recherche médicale (INSERM), Centre Léon Bérard, Université Lyon 1, Université Lyon, 3Biostatistics and Treatment Evaluation Unit, Centre Léon Bérard Here, we present an ablate-and-resect prospective study to evaluate the feasibility, safety, and accuracy of intraoperative high-intensity focused ultrasound ablation in patients undergoing hepatectomy for liver metastases. Biology Isolation and Culture of Mouse Primary Pancreatic Acinar Cells Johann Gout1,2,3,4,5, Roxane M. Pommier1,2,3,4,5, David F. Vincent1,2,3,4,5, Bastien Kaniewski1,2,3,4,5, Sylvie Martel1,2,3,4,5, Ulrich Valcourt*1,2,3,4,5, Laurent Bartholin*1,2,3,4,5 1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.
