ICube Laboratoire des sciences de l'ingenieur, de l'informatique et de l'imagerie, UNISTRA/CNRS/ENGE 1 article published in JoVE Environment Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging Claire Villette1, Loïc Maurer1,2, Dimitri Heintz1 1Plant Imaging and Mass Spectrometry (PIMS), Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 2 This method uses mass spectrometry imaging (MSI) to understand metabolic processes in S. alba leaves when exposed to xenobiotics. The method allows the spatial localization of compounds of interest and their predicted metabolites within specific, intact tissues.