INSERM U932 1 article published in JoVE Bioengineering Traction Force Microscopy to Study B Lymphocyte Activation Anita Kumari1, Judith Pineau1, Ana-Maria Lennon-Duménil1, Martial Balland2, Paolo Pierobon1 1Institut Curie, PSL Research University, INSERM U932, 2Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1) Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.
