Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021 1 article published in JoVE Biology FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria. Hanna Manko1, Vincent Normant2,3, Quentin Perraud2,3, Tania Steffan1, Véronique Gasser2,3, Emmanuel Boutant1, Éléonore Réal1, Isabelle J. Schalk2,3, Yves Mély1, Julien Godet1,4 1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.
