Alveole 1 article published in JoVE Cancer Research Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions Joris Guyon1, Pierre-Olivier Strale2,3, Irati Romero-Garmendia4, Andreas Bikfalvi1, Vincent Studer*2, Thomas Daubon*4 1University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, 2Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, 3Alvéole, Bordeaux, France, 4University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.