APHP-Sorbonne university 1 article published in JoVE Neuroscience Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures Cindy Bokobza*1, Alice Jacquens*1,2, Manuela Zinni1, Valérie Faivre1, Jennifer Hua1, David Guenoun1, Caroline Userovici1, Shyamala Mani1, Vincent Degos1,2, Pierre Gressens1, Juliette Van Steenwinckel1 1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48, Université de Paris, 2Department of anesthesia and critical care, APHP-Sorbonne university Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.
