Lille Neuroscience and Cognition research centre 1 article published in JoVE Biology Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets Geetika Raizada1, Balasubramaniam Namasivayam2, Sameh Obeid3, Benjamin Brunel1, Wilfrid Boireau1, Eric Lesniewska4, Celine Elie-Caille1 1Université de Franche-Comté, CNRS UMR-6174, FEMTO-ST Institute, 2Lille Neuroscience & Cognition research centre, 3Institut Galien Paris-Saclay, CNRS UMR-8612 , Université Paris-Saclay, 4Interdisciplinary Lab Carnot Bourgogne LICB, CNRS UMR-6303, Université de Bourgogne Franche-Comté This paper proposes a new generation of multiparametric analytical platforms with increased throughput for the characterization of extracellular vesicle subsets. The method is based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy, coupled with Raman spectroscopy, to qualify vesicular targets trapped on a microarray biochip.
