Universite de Rennes 2 articles published in JoVE Developmental Biology Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila Emma Leroux1, Nourhene Ammar1, Savannah Moinet1, Thierry Pecot2, Hadi Boukhatmi1 1Institut de Génétique et Développement de Rennes (IGDR), UMR6290 CNRS - Université de Rennes, 2BIOSIT, UAR 3480 US 018, Université de Rennes Drosophila is a well-established model for studying key molecules that regulate myogenesis. However, current methods are insufficient to determine mRNA transcriptional dynamics and spatial distribution within syncytia. To address this limitation, we optimized an RNA fluorescence in situ hybridization method allowing the detection and quantification of mRNAs at a single-molecule scale. Biology Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers Eloïse Mussard1,2, Corinne Lencina1, Gaëlle Boudry3, Caroline S. Achard2, Christian Klotz4, Sylvie Combes1, Martin Beaumont1 1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.
