Aintree University Hospital NHS Foundation Trust 1 article published in JoVE Immunology and Infection Quantification of Efferocytosis by Single-cell Fluorescence Microscopy Kyle Taruc1, Charles Yin1, Daniel G. Wootton2,3, Bryan Heit1 1Department of Microbiology and Immunology and the Center for Human Immunology, University of Western Ontario, 2Institute of Infection and Global Health, University of Liverpool, 3Department of Respiratory Research, Aintree University Hospital NHS Foundation Trust Efferocytosis, the phagocytic removal of apoptotic cells, is required to maintain homeostasis and is facilitated by receptors and signaling pathways that allow for the recognition, engulfment, and internalization of apoptotic cells. Herein, we present a fluorescence microscopy protocol for the quantification of efferocytosis and the activity of efferocytic signaling pathways.
