The London School of Hygiene & Tropical Medicine (LSHTM) 4 articles published in JoVE Immunology and Infection Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis Thomas Labadie1, Quentin Grassin2, Christophe Batéjat2, Jean-Claude Manuguerra2, India Leclercq2,3 1Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), 3Cellule Pasteur, Université de Paris Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions. Medicine An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay Lien Anh Ha Do1,2, Reuben Tse1, Jordan Nathanielsz1, Jeremy Anderson1, Darren Suryawijaya Ong1, Keith Chappell3, Kim Mulholland1,2,4, Paul V. Licciardi1,2 1 This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types. Immunology and Infection Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis Hollie Burrell-Saward1, Theresa H. Ward1 1Department of Infection and Immunity, London School of Hygiene & Tropical Diseases This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in real-time. Immunology and Infection Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model Neveda Naz1, Dominic C. Mills1, Brendan W. Wren1, Nick Dorrell1 1Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.
