Sheffield Teaching Hospitals NHS Foundation Trust 1 article published in JoVE Bioengineering Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model Nicola H. Green1, Bernard M. Corfe2, Jonathan P. Bury3, Sheila MacNeil1 1Department of Materials Science and Engineering, University of Sheffield, 2Department of Oncology and Insigneo Institute for in silico Medicine, University of Sheffield, 3Department of Histopathology, Sheffield Teaching Hospitals NHS Foundation Trust This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.