Diamond Lightsource 2 articles published in JoVE Biology Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells Nina Vyas*1, Nina Perry*1, Chidinma A. Okolo1, Ilias Kounatidis1, Thomas M. Fish1, Kamal L. Nahas1,2, Archana Jadhav1, Mohamed A. Koronfel1, Johannes Groen3, Eva Pereiro3, Ian M. Dobbie4, Maria Harkiolaki1 1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells. Engineering Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography Hannah P. Menke1, Matthew G. Andrew2, Joan Vila-Comamala3, Christoph Rau3, Martin J. Blunt1, Branko Bijeljic1 1Department of Earth Science and Engineering, Imperial College London, 2Carl Zeiss X-Ray Microscopy, 3Diamond Manchester Imaging Branchline (I13-2), Diamond Lightsource Synchrotron fast tomography was used to dynamically image dissolution of limestone in the presence of CO2-saturated brine at reservoir conditions. 100 scans were taken at a 6.1 µm resolution over a period of 2 h.
