Moorfields Eye Hospital 1 article published in JoVE Medicine Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres Shannon Bunker1, Joanna Holeniewska1, Sauparnika Vijay2, Annegret Dahlmann-Noor2,3, Peng Khaw2,4, Yin-Shan Ng5, David Shima1,6, Richard Foxton1 1Ocular Biology and Therapeutics, University College London Institute of Ophthalmology, 2University College London Institue of Ophthalmology, 3Moorfields Eye Hospital, 4NIHR Biomedical Research Centre, Moorfields Eye Hospital, 5Schepens Eye Research Institute, Harvard Medical School, 6Hoffman-La Roche We present a method for inducing elevated intraocular pressure (IOP), by injecting magnetic microspheres into the rat eye, to model glaucoma. This leads to strong pressure rises, and extensive neuronal death. This protocol is easy to perform, does not require repeat injections, and produces stable long-lasting IOP rises.
