Institute of Cancer Research, London 1 article published in JoVE Medicine Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts Marc D. Bullock1,2, Max Mellone1, Karen M. Pickard1, Abdulkadir Emre Sayan1, Richard Mitter3, John N. Primrose2, Graham K. Packham1, Gareth Thomas1, Alexander H. Mirnezami1,2 1Cancer Sciences Unit, University of Southampton School of Medicine, 2University Surgical Unit, University of Southampton School of Medicine, 3Bioinformatics Unit, London Research Institute, Cancer Research UK Molecular profiling of laser microdissected cells and stroma from synthetic, tissue-engineered colorectal cancer models represents a novel and manipulatable approach to characterize and dissect the distinctive biology at the interface between tumor cells at the invasive front and cancer associated stromal cells.
