The Pirbright Institute and UK-China Centre of Excellence for Research on Avian Diseases 1 article published in JoVE Immunology and Infection Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing Na Tang1,2, Yaoyao Zhang1, Miriam Pedrera1, Pengxiang Chang1, Susan Baigent1, Katy Moffat1, Zhiqiang Shen2, Venugopal Nair1, Yongxiu Yao1 1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.
