Animal and Plant Health Agency (APHA) 1 article published in JoVE Immunology and Infection Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis Denise A. Marston1, Daisy L. Jennings1, Nikki C. MacLaren1, Daniel Dorey-Robinson1, Anthony R. Fooks1,2, Ashley C. Banyard1, Lorraine M. McElhinney1,2 1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.
