Babraham Institute 3 articles published in JoVE Biology Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence Felipe J. Renna1, Malena Herrera Lopez1, Maria Manifava2, Nicholas T. Ktistakis2, Maria I. Vaccaro1 1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification. Biology Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans Hanneke Okkenhaug1, Laetitia Chauve1, Fatemeh Masoudzadeh1, Lars Okkenhaug1, Olivia Casanueva1 1Babraham Institute Worm-align/Worm_CP is a simple FIJI/CellProfiler workflow that can be used to straighten and align Caenorhabditis elegans samples and to score whole-worm image-based assays without the need for prior training steps. We have applied Worm-align/Worm_CP to the quantification of heat-shock induced expression in live animals or lipid droplets in fixed samples. Genetics High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology Laetitia Chauve*1, Jérémie Le Pen*2,3,5, Francesca Hodge1, Pia Todtenhaupt1, Laura Biggins1, Eric A. Miska2,3,4, Simon Andrews1, Olivia Casanueva1 1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University In this article a high-throughput protocol for fast and reliable determination of gene expression levels in single or bulk C. elegans samples is described. This protocol does not require RNA isolation and produces cDNA directly from samples. It can be used together with high-throughput multiplexed nanofluidic real-time qPCR platforms.