Harwell Campus 1 article published in JoVE Biochemistry A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline Adam D. Crawshaw1, Emma V. Beale1,2, Anna J. Warren1, Andrew Stallwood1,3, Graham Duller1, Jose Trincao1, Gwyndaf Evans1,4 1Diamond Light Source Ltd, Harwell Science and Innovation Campus, 2Paul Scherrer Institut, 3Central Laser Facility, Science and Technologies Facilities Council, Harwell Science and Innovation Campus, 4Rosalind Franklin Institute, Harwell Science and Innovation Campus The signal-to-noise ratio of data is one of the most important considerations in performing X-ray diffraction measurements from microcrystals. The VMXm beamline provides a low-noise environment and microbeam for such experiments. Here, we describe sample preparation methods for mounting and cooling microcrystals for VMXm and other microfocus macromolecular crystallography beamlines.
