University of Cambridge School of Clinical Medicine 1 article published in JoVE Immunology and Infection Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry Delphine M. Depierreux*1,2, Emily Seshadri*1,2, Evgeniya V. Shmeleva*1,2, Jens Kieckbusch1,2, Delia A. Hawkes1, Francesco Colucci1,2 1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research, University of Cambridge This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.
