Imperial College London and the University of Surrey 1 article published in JoVE Neuroscience Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology Adrien Rapeaux1,2,3,4, Omaer Syed5, Estelle Cuttaz5, Christopher A. R. Chapman5, Rylie A. Green5, Timothy G. Constandinou1,2,3,4 1Department of Electrical and Electronic Engineering, Imperial College London, 2Centre for Bioinspired Technology, Imperial College London, 3Care Research and Technology (CR&T), Imperial College London and the University of Surrey, 4Dementia Institute (UK DRI), 5Department of Bioengineering, Imperial College London This protocol describes the preparation of rat whole sciatic nerve tissue for ex vivo electrophysiological stimulation and recording in an environmentally-regulated, two-compartment, perfused saline bath.
