The University of Dublin 2 articles published in JoVE Immunology and Infection An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis Seónadh O’Leary1, Ahmad Z. Bahlool1,2, Gemma O’Connor2, Sally-Ann Cryan2,3,4, Joseph M. Keane1, Mary P. O’Sullivan1 1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies. Neuroscience Measurement & Analysis of the Temporal Discrimination Threshold Applied to Cervical Dystonia Rebecca B Beck*1, Eavan M McGovern*1,2,3, John S Butler4, Dorina Birsanu1, Brendan Quinlivan1, Ines Beiser1,2,3, Shruti Narasimham1, Sean O'Riordan2,3, Michael Hutchinson2,3, Richard B Reilly1,5 1School of Engineering, Trinity College Dublin, The University of Dublin, 2 Methods for the measurement and analysis of the temporal discrimination threshold are presented, and its application to the study of the pathogenesis of cervical dystonia are discussed.
