Fondazione IRCCS Istituto Nazionale dei Tumori 1 article published in JoVE Cancer Research MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening Mavis Mensah*1, Cristina Borzi*1, Carla Verri1, Paola Suatoni2, Davide Conte1, Ugo Pastorino2, Fortunato Orazio1, Gabriella Sozzi1, Mattia Boeri1 1Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 2Unit of Thoracic Surgery, Fondazione IRCCS Istituto Nazionale dei Tumori Here, we present the detailed protocol adopted in the BioMILD screening trial to perform the circulating microRNA signature classifier test for early lung cancer detection.