Centro Cardiologico Monzino-IRCCS 2 articles published in JoVE Biology Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples Roberto Plebani1,2, Alessandra D'Alessandro2, Paola Lanuti2,3, Pasquale Simeone2,3, Massimiliano Cinalli1, Ilaria Righi4, Alessandro Palleschi4,5, Matteo Mucci1,2, Marco Marchisio2,3, Francesco Cappabianca1, Marina Camera6,7, Felice Mucilli1, Mario Romano1,2 1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4 Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells. Developmental Biology Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients Chiara Assunta Pilato*1,2, Ilaria Stadiotti*1, Angela Serena Maione1, Valentina Saverio1, Valentina Catto3, Fabrizio Tundo3, Antonio Dello Russo3, Claudio Tondo2,3, Giulio Pompilio1,2, Michela Casella*3, Elena Sommariva*1 1Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health, Università degli Studi di Milano, 3Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.