IFOM, FIRC Institute of Molecular Oncology 1 article published in JoVE Medicine From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia Benedetta Apollonio1,2, Maria Teresa Sabrina Bertilaccio1, Umberto Restuccia3, Pamela Ranghetti1, Federica Barbaglio1, Paolo Ghia1,4, Federico Caligaris-Cappio1,4, Cristina Scielzo1 1Division of Molecular Oncology, IRCCS, San Raffaele Scientific Institute, 2 Here we describe a protocol that couples two proteomic techniques, namely 2-dimensional Electrophoresis (2DE) and Mass Spectrometry (MS), to identify differentially expressed/post-translational modified proteins among two or more groups of primary samples. This approach, together with functional experiments, allows the identification and characterization of prognostic markers/therapeutic targets.
