University of Catania 2 articles published in JoVE Biology Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii Marco Valerio Rossi-Stacconi1, Xingeng Wang2, Amanda Stout2, Lorenzo Fellin1,3, Kent M. Daane4, Antonio Biondi5, Judith M. Stahl4, Matthew L. Buffington6, Gianfranco Anfora1,3, Kim A. Hoelmer2 1Research and Innovation Centre, Fondazione Edmund Mach, 2Beneficial Insects Introduction Research Unit, Agricultural Research Service, United States Department of Agriculture, 3Center for Agriculture, Food and Environment, University of Trento, 4Department of Environmental Science, Policy and Management, University of California Berkeley, 5Department of Agriculture, Food and Environment, University of Catania, 6Systematic Entomology Laboratory, Agricultural Research Service, United States Department of Agriculture Ganaspis brasiliensis-a larval parasitoid of Drosophila suzukii (a global invasive fruit crop pest)-has been approved or is considered for introduction into Europe and the United States for biological control of this pest. This article provides protocols for both small-scale and large-scale rearing of this parasitoid. Neuroscience Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration María Portela-Lomba1,3, Diana Simón1, Cristina Russo2, Javier Sierra1, María Teresa Moreno-Flores3 1Facultad de CC Experimentales, Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers.
