Vita-Salute San Raffaele University 2 articles published in JoVE Bioengineering In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing Alessandro Migliara1, Martino Alfredo Cappelluti1, Francesca Giannese2, Sara Valsoni1, Alberto Coglot1, Ivan Merelli1,3, Davide Cittaro2, Angelo Lombardo1,4 1San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, 2Center for Omics Sciences, IRCCS San Raffaele Institute, 3Institute for Biomedical Technologies, National Research Council, 4Vita-Salute San Raffaele University Here, we present a protocol for the in vitro selection of engineered transcriptional repressors (ETRs) with high, long-term, stable, on-target silencing efficiency and low genome-wide, off-target activity. This workflow allows for reducing an initial, complex repertoire of candidate ETRs to a short list, suitable for further evaluation in therapeutically relevant settings. Medicine Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome Valeria Stella Vanni*1,2, Alessandra Alteri*1,2, Lucia De Santis1,2, Greta Chiara Cermisoni1, Elisa Rabellotti1, Diana Delprato1, Marta Parma1, Enrico Papaleo1, Luigi Fedele3, Massimo Candiani1,2 1Obstetrics and Gynaecology Unit, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3 A dedicated team can offer Mayer-Rokitansky-Kuster-Hauser patients the option to perform controlled ovarian stimulation and oocyte cryopreservation at the time of laparoscopic vaginoplasty.
