Confocal.nl BV 1 article published in JoVE Bioengineering Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores Juan Wen1, Raymond Pasman1, Erik M.M. Manders*2,3, Peter Setlow*4, Stanley Brul*1 1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics, UConn Health Germinant receptor proteins cluster in ‘germinosomes’ in the inner membrane of Bacillus subtilis spores. We describe a protocol using super resolution microscopy and fluorescent reporter proteins to visualize germinosomes. The protocol also identifies spore inner membrane domains that are preferentially stained with the membrane dye FM4-64.
