Amsterdam UMC 5 articles published in JoVE Biology High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System Leander A. Vonk*1, Osman Esen*1, Michaela Yuen1,4, Tyler J. Kirby1,2,3 1Department of Physiology, Amsterdam UMC, 2Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias, Amsterdam UMC, 3Amsterdam Movement Sciences, Tissue Function and Regeneration, Amsterdam UMC, 4Discipline of Child and Adolescent Health, Faculty of Health and Medicine, University of Sydney Skeletal muscle function can be assessed by quantifying the contractility of isolated muscle fibers, traditionally using laborious, low-throughput approaches. Here, we describe an optics-based, high-throughput method to quantify the contractility of hydrogel-embedded muscle fibers. This approach has applications for drug screening and therapeutic development. Medicine Robotic Left Hepatectomy using Indocyanine Green Fluorescence Imaging for an Intrahepatic Complex Biliary Cyst Burak Görgec1,2, Babs M. Zonderhuis2,3, Marc G. Besselink1,2, Joris Erdmann1,2,3, Geert Kazemier2,3, Rutger-Jan Swijnenburg1,2,3 1Department of Surgery, Amsterdam UMC, location University of Amsterdam, 2Cancer Center Amsterdam, 3Department of Surgery, Amsterdam UMC, location Vrije Universiteit Robotic liver surgery has gained more acceptance as a feasible, safe, and effective procedure for the treatment of both benign and malignant indications. However, robotic left hepatectomy is still technically demanding. We describe our surgical technique of a robotic left hepatectomy using indocyanine green fluorescence imaging for a large biliary cyst. Cancer Research Robotic Pancreatoduodenectomy for Pancreatic Head Cancer: a Case Report of a Standardized Technique Maurice J. W. Zwart*1,2, Leia R. Jones*1,2,3, Melissa E. Hogg4, Johanna A. M. G. Tol1,2, Mohammad Abu Hilal3, Freek Daams5, Sebastiaan Festen6, Olivier R. Busch1,2, Marc G. Besselink1,2, HPB-Amsterdam, 1Department of Surgery, Amsterdam, the Netherlands, Amsterdam UMC, location University of Amsterdam, 2Cancer Center Amsterdam, the Netherlands, 3Department of General Surgery, Istituto Ospedaliero Fondazione Poliambulanza, 4Department of Surgery, Northshore University Health System, 5Department of Surgery, Amsterdam UMC, Vrije Universiteit, Cancer Center Amsterdam, 6Department of Surgery, OLVG Robotic pancreatoduodenctomy (RPD) has been highly standardized in recent years and may be used in selected patients with pancreatic head cancer, including those with a replaced right hepatic artery. This case report describes a standardized and reproducible technique for RPD, which includes the approach of the Dutch LAELAPS-3 training program to an aberrant vasculature. Medicine Acetylcholine Re-Challenge After Intracoronary Nitroglycerine Administration Rutger G. T. Feenstra1, Tim P. van de Hoef1, Marcel A. M. Beijk1, Jan J. Piek1 1Heart Center, Department of Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC This protocol presents the acetylcholine rechallenge after nitroglycerine as an add-on procedure to spasm provocation testing. The purpose of this technique is to unmask co-existing microvascular spasm in patients with epicardial spasm and to assess the protective efficacy of nitroglycerine on a per-patient level to guide medical therapy. Biology Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer Martijn van de Locht1, Josine M. de Winter1, Dilson E. Rassier2, Michiel H.B. Helmes1,3, Coen A.C. Ottenheijm1 1Department of Physiology, Amsterdam UMC, 2Department of Kinesiology and Physical Education, Faculty of Education, McGill University, 3IONOptix BV Presented here is a protocol to assess the contractile properties of striated muscle myofibrils with nano-Newton resolution. The protocol employs a setup with an interferometry-based, optical force probe. This setup generates data with a high signal-to-noise ratio and enables the assessment of the contractile kinetics of myofibrils.
