Oncode Institute 3 articles published in JoVE Biology Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling Dimitrios Laskaris*1,2, Maria Azkanaz*1,2, Mijke A. de Vreij-Kruidenier3, Doreen van Rijswoud-Ram3, Hendrik A. Messal1,2, Jacco van Rheenen1,2 1Department of Molecular Pathology, The Netherlands Cancer Institute, 2Oncode Institute, 3Animal Laboratory Facility, The Netherlands Cancer Institute Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is captured. Biology Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid Larissa Mourao1,2, Marta Ciwinska1,2, Jacco van Rheenen3, Colinda L. G. J. Scheele1,2 1Center for Cancer Biology, VIB (BE), 2Department of Oncology, KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL) This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages. Biology Generation of Human Kidney Tubuloids from Tissue and Urine Camilla Calandrini1,2, Jarno Drost1,2 1Princess Máxima Center for Pediatric Oncology, 2Oncode Institute Human kidney tubuloid cultures represent a valuable in vitro model to study kidney physiology and disease. Tubuloids can be established from kidney tissue (healthy and diseased) as well as urine, the latter representing an easily obtainable and less invasive source of research material.
