Norwegian Veterinary Institute 1 article published in JoVE Genetics Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis Snorre Gulla1, Saima Nasrin Mohammad1, Duncan John Colquhoun1,2 1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.
