Silesian Centre for Heart Diseases 1 article published in JoVE Immunology and Infection Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization Anna Kula-Pacurar1, Jakub Wadas2, Agnieszka Suder1, Artur Szczepanski1,2, Aleksandra Milewska1,2, Marek Ochman3,4, Tomasz Stacel3,4, Krzysztof Pyrc1 1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.
