Champalimaud Center for the Unknown View Institution's Website 2 articles published in JoVE Cancer Research Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis Mayra Martinez-Lopez1,2, Vanda Póvoa1, Rita Fior1 1Champalimaud Centre for the Unknown, Champalimaud Foundation, 2Instituto Gulbenkian de Ciência Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification. Neuroscience In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons Vania Y. Cao1,2, Yizhou Ye1, Surjeet S. Mastwal1, David M. Lovinger3, Rui M. Costa4, Kuan H. Wang1 1Unit on Neural Circuits and Adaptive Behaviors, Genes Cognition and Psychosis Program, National Institute of Mental Health, 2Department of Neuroscience, Brown University - National Institutes of Health Graduate Partnership Program, 3Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, 4Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown Experience-dependent molecular changes in neurons are essential for the brain's ability to adapt in response to behavioral challenges. An in vivo two-photon imaging method is described here that allows the tracking of such molecular changes in individual cortical neurons through genetically encoded reporters.
