Universidade do Minho 1 article published in JoVE Biology Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique Guillaume Luxardi1, Brian Reid1, Fernando Ferreira1,2, Pauline Maillard3, Min Zhao1,4 1Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, 2Departamento de Biologia, Centro de Biologia Molecular e Ambiental, Universidade do Minho, 3Department of Neurology and Center for Neuroscience, University of California, Davis Imaging of Dementia and Aging Laboratory, 4Department of Ophthalmology, Institute for Regenerative Cures, University of California, Davis Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.
