Centro Hospitalar Universitario Lisboa Norte 3 articles published in JoVE Medicine Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model Pedro S. de Oliveira1,2, Filipe Rocha1, Inês Sofia Vala1, Paula de Oliveira1, Augusto Ministro1,3, Susana Constantino Rosa Santos1 1Cardiovascular Center of the University of Lisbon (CCUL@RISE), Faculdade de Medicina da Universidade de Lisboa, 2Department of Urology, Centro Hospitalar Universitário Lisboa Norte, 3Department of Vascular Surgery, Centro Hospitalar Universitário Lisboa Norte In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement. Medicine Echocardiographic Assessment of Cardiac Anatomy and Function in Adult Rats Sónia Ribeiro*1,2, Ana Rita S. Pereira*1, Ana Teresa Pinto1, Filipe Rocha1, Augusto Ministro1,2, Manuela Fiuza1,2, Fausto Pinto1,2, Susana Constantino Rosa Santos1 1Centro Cardiovascular da Universidade de Lisboa, Faculdade de Medicina, Universidade de Lisboa, 2Departamento Coração e Vasos, Centro Hospitalar Universitário Lisboa Norte A non-invasive protocol for transthoracic echocardiography assessment of cardiac anatomy and function for adult rats is presented in the current study. The heart valves, all four cardiac chambers and the ascending aorta, aortic arch and descending aorta are studied in detail. Medicine Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia Augusto Ministro1,2, Paula de Oliveira1, Raquel J. Nunes1, André dos Santos Rocha1, Tiago Ferreira2, J. Goyri-O'Neill3, Susana Constantino Rosa Santos1 1Centro Cardiovascular da Universidade de Lisboa, Lisbon School of Medicine of the Universidade de Lisboa, 2Centro Hospitalar Universitário Lisboa Norte, 3Nova Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa Here, a critical hindlimb ischemia experimental model is presented followed by a battery of functional, histologic and molecular tests to assess the effectiveness of angiogenic therapies.
