Porto Comprehensive Cancer Center (P.CCC) 1 article published in JoVE Biology Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells Laura Mosteo1, Joana Reis1, Lídia Rocha1, Marta Lopes1, Delfim Duarte1,2,3,4 1Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 2Department of Onco-Hematology, Instituto Português de Oncologia (IPO)-Porto, 3Unit of Biochemistry, Department of Biomedicine, Faculdade de Medicina da Universidade do Porto, 4Porto Comprehensive Cancer Center (P.CCC) Here, we describe a simple protocol for the isolation and staining of murine bone marrow cells to phenotype hemopoietic stem and progenitor cells along with the supporting niche endothelial and mesenchymal stem cells. A method to enrich cells located in endosteal and central bone marrow areas is also included.
