University of Belgrade Faculty of Biology 1 article published in JoVE Neuroscience Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis Katarina Milićević1, Andrej Korenić1, Milena Milošević1, Pavle R. Andjus1 1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.
