Sahlgrenska University Hospital 4 articles published in JoVE Bioengineering Isolation and Decellularization of a Whole Porcine Pancreas Vijay Kumar Kuna*1, Niclas Kvarnström*2, Erik Elebring1, Suchitra Sumitran Holgersson1 1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 2The Transplant Institute, Sahlgrenska University Hospital Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease. Neuroscience Kinematic Analysis Using 3D Motion Capture of Drinking Task in People With and Without Upper-extremity Impairments Margit Alt Murphy1,3, Steve Murphy2, Hanna C. Persson1,3, Ulla-Britt Bergström3, Katharina Stibrant Sunnerhagen1 1Institution of Neuroscience and Physiology, Rehabilitation Medicine, Sahlgrenska Academy, University of Gothenburg, 2Securesoft Sweden, 3Departement of Occupational Therapy and Physiotherapy, Sahlgrenska University Hospital This protocol describes an objective method to evaluate the movement performance and sensorimotor function of the upper extremity applied to individuals with stroke and healthy controls. A standardized test procedure, kinematic analysis and outcome variables for three-dimensional motion capture of drinking task are provided. Neuroscience Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid Karl T. Hansson1, Tobias Skillbäck1,2, Elin Pernevik1, Jessica Holmén-Larsson1, Gunnar Brinkmalm1, Kaj Blennow1,2, Henrik Zetterberg1,2,3, Johan Gobom1,2 1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3Department of Molecular Neuroscience, UCL Institute of Neurology A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies. Medicine Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure Josef Pannee1,2, Kaj Blennow1,2, Henrik Zetterberg1,2,3, Erik Portelius1,2 1Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3UCL Institute of Neurology, Queen Square A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.
