Karolinska Institute 1 article published in JoVE Biology Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches Dmitry K. Nilov1, Alexey V. Zamaraev2, Boris Zhivotovsky2,3, Gelina S. Kopeina2 1Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University, 2Faculty of Medicine, Lomonosov Moscow State University, 3Division of Toxicology, Institute of Environmental Medicine, Karolinska Institute The present protocol uses a biomolecular simulation package and describes the molecular dynamics (MD) approach for modeling the wild-type caspase and its mutant forms. The MD method allows for assessing the dynamic evolution of the caspase structure and the potential effect of mutations or post-translational modifications.
