Lund University Hospital 1 article published in JoVE Neuroscience Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders Ana Gonzalez-Ramos2, Kerstin Laurin1, Fredrik Berglind1, Marco Ledri3, Merab Kokaia2, My Andersson1 1Cellular Neurophysiology and Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 2Experimental Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 3Molecular Neurophysiology and Epilepsy Group, Epilepsy Center, Department of Clinical Sciences, Lund University Hospital Transplantation of human pluripotent stem cell-derived GABAergic neurons generated by neuronal programming could be a potential treatment approach for neurodevelopmental disorders. This protocol describes the generation and transplantation of human stem cell-derived GABAergic neuronal precursors into the brains of neonatal mice, allowing long-term investigation of grafted neurons and evaluation of their therapeutic potential.