University Medical Centre Ljubljana 1 article published in JoVE Genetics Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis Tadej Pajič1,2, Tanja Belčič Mikič1, Helena Podgornik1,3, Jurka Klun1, Sandra Šućurović1, Samo Zver1, Matjaz Sever1 1Department of Hematology, University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine, University Medical Centre Ljubljana, 3Faculty of Pharmacy, University of Ljubljana High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.
