2 articles published in JoVE
Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil Stefan Wagner1, Christoph Hoefer2, Thomas Prohaska1, Jakob Santner1,3 1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry, Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group, University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy, University of Natural Resources and Life Sciences, Vienna This protocol presents a workflow for sub-mm 2D visualization of multiple labile inorganic nutrient and contaminant solute species using diffusive gradients in thin films (DGT) combined with mass spectrometry imaging. Solute sampling and high-resolution chemical analysis are described in detail for quantitative mapping of solutes in the rhizosphere of terrestrial plants.
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols Gernot Bodner1, Mouhannad Alsalem1, Alireza Nakhforoosh1, Thomas Arnold2, Daniel Leitner3,4 1Division of Agronomy, Department of Crop Sciences, University of Natural Resources and Life Sciences, 2Carinthian Tech Research AG, High Tech Campus Villach, 3Computational Science Center, University of Vienna, 4Simulationswerkstatt An experimental protocol is presented for assessment of soil grown plant root systems with RGB and hyperspectral imaging. Combination of RGB image time series with chemometric information from hyperspectral scans optimizes insights into plant root dynamics.